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Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can provide information about dynamic biological processes.〔〔 According to the Washington Post, Betzig believes that this development will have a greater impact than the work that earned him a Nobel Prize.〔 It was developed in the early 2010s by Eric Betzig,〔 who also won the 2014 Nobel Prize in Chemistry for "the development of super-resolved fluorescence microscopy". == Setup of Lattice Light-sheet Fluorescence Microscopy == Lattice light sheet microscopy is a novel combination of techniques from Light sheet fluorescence microscopy, Bessel beam microscopy, and Super-resolution microscopy specifically Structured illumination microscopy (SIM). In lattice light sheet microscopy, very similar to light sheet microscopy the illumination of the sample occurs perpendicular to the image detection. Initially the light sheet is formed by stretching the linearly polarized circular input beam with a pair of cylindrical lenses along the x axis and then compressing it with an additional pair of lenses along the z axis.〔 This modification creates a thin sheet of light that is then projected onto a binary ferroelectric spatial light modulator (SLM). The SLM is a device that spatially varies the waveform of a beam of light. The light that is reflected back from the SLM is used to eliminate unwanted diffraction. Diffraction is eliminated by the transform lens that creates a Fraunhofer diffraction pattern from the reflected light at an opaque mask containing a transparent annulus.〔 Optical lattices are two or three dimensional interference patterns, which here are produced by the transparent annular ring. The mask is conjugate to x and z galvanometers. This quality of the microscope is important for the dithered mode of operation, where the light sheet must be oscillated within the x axis. The lattice light-sheet microscope has two modes of operation: In the dithered mode, the light sheet is rapidly scanned along the x axis and only one image is recorded per Z plane, at normal diffraction limited resolutions.〔 The second mode of operation is the structured illumination microscopy mode (SIM). SIM is a technique where a grid pattern of excitation light is superimposed on the sample and rotated in steps between the capture of each image.〔 These images are then processed via an algorithm to produce a reconstructed image past the limit of diffraction that is built into our optical instruments. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Lattice light-sheet microscopy」の詳細全文を読む スポンサード リンク
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